Syllabi of

Core Courses

Core Course- 1                                            

MB1B01U Fundamentals of Microbiology

Total hours of instruction 72

Hours per week 4

Credits: 4

Unit 1

 Definition, Scope and history of microbiology.  Beneficial and harmful microbes. Diversity of microbial world. Principles of classification- classification of bacteria & algae. Ultrastructure of prokaryotic cell.  Differentiate between eukaryote and prokaryote.  Differences between archaebacteria and eubacteria.

References

Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K. Jayaram Panikar

Microbiology by Daniel Lim

Microbiology: Principles and Explorations by Jacquelyn G. Black

Prescott/Harley/Klein's Microbiology by Joanne Willey, Linda Sherwood, and Chris Woolverton

Microbiology  Pelczar, Chan and Krieg.

Unit 2

Microscopes, Staining techniques, Culture media and culture methods (including anaerobic bacteria)  Isolation of pure cultures.

 References:

.Biophysics  R.N.Roy  

General microbiology Vol 1 Powar & Daginawala

 Microbiology  Pelczar, Chan and Krieg.

 Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K  Jayaram Panikar

Unit 3

Sterilisation and disinfection – physical and chemical methods. Antimicrobial agents –mode of action

References:

 Microbiology  Pelczar, Chan and Krieg.. Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K. Jayaram Panikar

.Prescott/Harley/Klein's Microbiology by Joanne Willey, Linda Sherwood, and Chris Woolverton

 General microbiology Vol 1 Powar & Daginawala

Unit 4: Nutrition, nutritional types, growth requirements and conditions influencing growth, growth curve, growth kinetics, phases of growth, cell division, sporulation, germination. Enumeration & quantitation of bacteria and microbes. Preservation and transport of bacteria.

 References:

 Microbiology  Pelczar, Chan and Krieg.

 Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K. Jayaram Panikar

  Prescott/Harley/Klein's Microbiology by Joanne Willey, Linda Sherwood, and Chris Woolverton

 General microbiology Vol 1 Powar & Daginawala

Core Course- 2

MB1B02U  Microbial metabolism and Microbial genetics

Total hours of  instruction 72

Hours per week 4

Credits: 4

Unit 1 : Bacterial metabolism, enzymes, factors affecting enzyme activity, transition state in enzyme catalysed reactions, high energy compounds- ATP, GTP, role of reducing power of NAD, NADPH.

References:

General microbiology Vol 1 Powar & Daginawala

General microbiology Vol 2 Powar & Daginawala

Microbiology  Pelczar, Chan and Krieg

Unit 2 : Carbohydrate metabolism, glycolysis, alcoholic fermentation, TCA cycle, glyoxalate cycle, electron transport chain, substrate level and oxidative phosphorylation, pentose phosphate pathway. Photosynthesis , nitrogen fixation . Transamination and methanogenesis.

References:

General microbiology Vol 1 Powar & Daginawala

General microbiology Vol 2 Powar & Daginawala

 Microbiology  Pelczar, Chan and Krieg

 Prescott/Harley/Klein's Microbiology by Joanne Willey, Linda Sherwood, and Chris Woolverton

Unit 3: Bacterial chromosome- structure, replication, extrachromosomal genetic material. Plasmids – structure, replication, incompatibility, plasmid amplification. Transposons –types, transposition.  Genetic exchange – conjugation, transformation, transduction. Mechanism and spread of antibiotic resistance in bacteria.

References:

 Microbial Genetics Stanley R. Maloy, Freifelder and Cronan

 Molecular Genetics of Bacteria Snyder and Charminess.

Fundamentals of molecular Biology  by Veer Bala Rastogi Ane books India

Unit 4: DNA repair & restriction, types of repair systems.  Gene expression – transcription, translation, control of gene expression in microbes.  Mutation- spontaneous, induced, base pair changes, frame shift, deletion, inversion, insertion, tandem duplications, useful phenotypes (auxotrophic, conditional, lethal, resistant) Reversion vs suppression, Ames test.

References

Microbial Genetics Stanley R. Maloy, Freifelder and Cronan

Molecular Genetics of Bacteria Snyder and Charminess.

Core Course -3

MB1B 03U Microbiology Practical – I

Total hours of instruction: 72        

hours/ week: 4             

Credit : 2

Study the parts and usage of a Compound Microscope

Study the parts and working and uses of

Autoclaves

Hot air oven

Membrane Filter

Safety Cabinet

Anaerobic Jar

Centrifuge

Incubator

Preparation of culture Media (NB, NA, MA) and dispensing media in test tubes, bottles, petridishes.

Cultivation of Bacteria on nutrient Agar for obtaining isolated colonies.Study of cultural colony characters- Size, shape, colour etc.

Viable Count of bacteria by pour plate/ spread plate method.

Examination of wet films under high power objectives.

Preparation and examination of Hanging drop mount for studying the motility of bacteria.

Preparation of slide smears for staining.

Staining- Principle & techniques           

- Simple staining

- Gram Staining

                                                - Negative Staining.

                                                - Special Staining – endospores, volutin granules

Microscopic study of Bacteria – Cocci, Bacilli

Preparation of Fungal media

Cultivation of fungi study of colony characters of yeast and mold.

References:

 Microbiology – Concepts and Application – Pelzer Jr. Chang Kreig Mac Graw Hill Inc

 Microbiology – Prescott, Harley and Klein Wim.C.Brown Publishers.

 Practical Microbiology – R.C Dubey, D.K Maheshwari, S Chand and Company, New Delhi.

 Microbiology Laboratory Manual – Cappuccino, Sherman, Pearson Education

Manual of Microbiology Kanika Sharma Ane Books Pvt. Ltd.

Core Course-4

MB2B04U Immunology        

Total Instruction hours- 72

Hours / week- 4

Credits- 4

Unit I:

History of Immunology, Introduction to Infection, Types of Immunity- innate immunity and acquired immunity, Cells and organs involved in immune system

References:

Immunology Janis Kuby, Thomas J. Kindt, Barbara A. Osborne, and Richard A. Goldsby

 Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K. Jayaram Panikar

.Kannan (2007), "Immunology",MJP Publishers, Chennai 600005.

Roitt's Essential Immunology by Peter Delves , Seamus Martin , Dennis Burton, Ivan Roitt

Unit II:

Antigens- types, properties, Haptens, Adjuvants; Vaccines - Types - Toxoids - antitoxins., Immunoglobulins- Structure, types and properties. Complement- structure properties, function of complement components and pathways, Major histocompatibility complex- HLA, H2

Antigen antibody reaction- precipitation reactions, agglutination reactions, complement fixation, Neutralisation reactions, Inmiunofluroscence, ELISA, RIA.

References:

Immunology Janis Kuby, Thomas J. Kindt, Barbara A. Osborne, and Richard A. Goldsby

 Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K. Jayaram Panikar

I.Kannan (2007), "Immunology",MJP Publishers, Chennai 600005.

Roitt's Essential Immunology by Peter Delves , Seamus Martin , Dennis Burton, Ivan Roitt

Unit III:

Humoral Immune response- Theories of antibody production, plasma cells and antibody secretion, Monoclonal antibodies, Cell mediated immune response- Cytokines, natural killer cells and antibody dependent cell mediated cytotoxicity

References:

Immunology Janis Kuby, Thomas J. Kindt, Barbara A. Osborne, and Richard A. Goldsby

Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K. Jayaram Panikar

.Kannan (2007), "Immunology",MJP Publishers, Chennai 600005.

Roitt's Essential Immunology by Peter Delves , Seamus Martin , Dennis Burton, Ivan Roitt

Unit IV:

Immunohaematology- Blood groups. Blood transfusion- Rh incompatibilities, Hypersensitivity Reactions- Type I, II, III, & IV. Brief of Transplantation Immunology, Autoimmunity, Tumor Immunology

References:

Immunology Janis Kuby, Thomas J. Kindt, Barbara A. Osborne, and Richard A. Goldsby

Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K. Jayaram Panikar

I.Kannan (2007), "Immunology",MJP Publishers, Chennai 600005.

Roitt's Essential Immunology by Peter Delves , Seamus Martin , Dennis Burton, Ivan Roitt

Core Course-5

MB2B05U  Science Methodology and Biostatistics

Unit I

Science and Tools of Science

Types of knowledge: practical, theoretical and scientific knowledge. Basis for Scientific laws and factual truths. Revolutions in Science and Technology.

Hypothesis; theories and laws in Science; observations, evidences and proofs. Significance of peer Review.

Reference:

Debbie Holmes, Peter Moody, Diana Dine.  Research methods for the biosciences, International student edition,Oxford University Press Inc.New York

S.K.Aggarwal. Foundation course in Biology, Ane’s Student Edition, 2^nd  edition

R.C. Sobti, V.L.Sharma. Essentials ofModern Biology, Ane’s Student Edition.

Unit II

Experimentation in Science and Data Handling

Design of an experiment; experimentation; observation; data collection; interpretation and deduction. Necessity of units and dimensions; repeatability and replication. Documentation of experiments, Record keeping. Connection between measurements and underlying theory.Documentation of experiments.

 Nature and types of data – typical examples; data acquisition; treatment of data; data interpretation. Significance of statistical tools in data interpretation: graphs, tables, histograms and pi diagrams.

Statistical testing of hypothesis, null hypothesis. Significance test – Statistics based acceptance or rejection of a hypothesis. Deduction of scientific correlation, patterns and trends.

Reference:

Debbie Holmes, Peter Moody, Diana Dine.  Research methods for the biosciences, International student edition,Oxford University Press Inc.New York

S.K.Aggarwal. Foundation course in Biology, Ane’s Student Edition, 2^nd  edition

R.C. Sobti, V.L.Sharma. Essentials ofModern Biology, Ane’s Student Edition.

Unit III

Nature and scope of statical methods and their limitation.

compilation,classification,tabulation, and application in life science.

Graphical representation,Measure of average and dispersion mean,medium,mode.

Reference

Fundamentals of Biostatistics. Bernard Rosner

Biostatistics for  medical,nursing and pharmacy students.a.indrayan and L.Satyanarayana.

Statistics for Biologists.Campbell.R.C

Unit IV

Sampling methods – simple random ,stratified, systematic and cluster sampling procedures. Sampling distribution,Probability, Tests of significance based on T, Chi-square and F Test Designing and methodology of experiment

Reference

Fundamentals of Biostatistics. Bernard Rosner

Biostatistics for  medical,nursing and pharmacy students.a.indrayan and L.Satyanarayana.

Statistics for Biologists.Campbell.R.C

Core Course- 6

MB2B06U Microbiology Practical – II

Total hours of instruction: 72 

Hours/week: 4 

Credit: 2

Slide agglutination test ,Blood grouping, ASO

Bacterial agglutination test

Precipitation reaction – RPR ,VDRL, , ODD,  

 RID,

 ELISA-Demonstration

 Titration of Antibody – Widal Test.

Reference

 Bailey and Scott’s Diagnostic Microbiology

 Practical Medical Microbiology by Mackie & Mc Cartney

 Hand book of experimental immunology by D.M. Weir

 Practical Microbiology by Dubey and Maheswari

 Experiments in Microbiology, Plant Pathology and Biotechnology by K.R. Aneja

Core Course- 7

MB3B07U  Industrial Microbiology

Total hours- 90

Hours / week- 5

Credits- 4

Unit I:

General concepts of industrial microbiology, principles of exploitation of microorganisms of their products. Introduction to microbial products obtained by industrial process-primary and secondary metabolites. Industrial strains– characteristics and isolation techniques- primary and secondary staining techniques, strain improvement.

References

Principles of Fermentation Technology by Peter F. Stanbury, Stephen J. Hall, and Allan Whitaker

Industrial Microbiology by L.E. Casida

Industrial and Environmental Biotechnology byNuzhat Ahmed ; Fouad M Qureshi and Obaid Y.Khan

Unit II:

Industrial fermentation equipment and its uses, types of fermentation -single, batch, continuous, dual or multiple, solid- state and submerged fermentation. Fermentation media formulation strategies, means of providing energy, carbon, nitrogen, vitamin and mineral sources, role of buffers, precursors, inhibitors, inducers and antifoams,  products recovery and purification.

 References

Principles of Fermentation Technology by Peter F. Stanbury, Stephen J. Hall, and Allan Whitaker

Industrial Microbiology by L.E. Casida

Unit III:

Industrial products derived from microbes, industrial Enzymes-amylase, proteinase, celluase, Aminoacid production - glutamic acid and lysine. Production of antibiotics pemcillins, streptomycins, Vitamins- ribofalvin, cyanocobalamin. Vaccines - genetic recombinant vaccines.

References

Manual of Industrial Microbiology and Biotechnology by Ronald M. Atlas

Prescott and Dunn's Industrial Microbiology by Gerald Reed

Industrial Microbiology by Samuel C. Prescott

Industrial Microbiology by L.E. Casida

Unit IV:

Immobilization methods, adsorption; covalent linkages, membrane entrapment- advantages and disadvantages,

Intellectural Property Rights (IPR)

IPR - Definition - WTO - Defmition - Functions - Forms of IPR Protection.

References

Principles of Fermentation Technology by Peter F. Stanbury, Stephen J. Hall, and Allan Whitaker

Manual of Industrial Microbiology and Biotechnology by Ronald M. Atlas

Prescott and Dunn's Industrial Microbiology by Gerald Reed

Industrial Microbiology by Samuel C. Prescott

Industrial Microbiology by L.E. Casida

Core Course-8

MB3 08U Soil microbiology

Total hours of instruction :72

Hours / week:4

Credits:4

Unit 1

Soil environment- soil structure, physico chemical characteristics, microbial composition and influence of soil and environmental factors on soil micro flora .Quantitative and qualitative estimation of microorganisms in soil. Organic matter decomposition, Methanogenesis  Humus formation.

References

1.Soil microbiology;An exploratory approach-Mark.s.coyne

2.Introduction to soil microbiology-Martin Alexander

3.Soil microorganisms and plant growth-N.S.Subba Rao

Unit 2

Major biogeochemical cycles and the organisms. Microbial transformation of carbon-nitrogen-phosphorus and sulphur. Microbes in composting and biogas production

References

1.Soil microbiology;An exploratory approach-Mark.s.coyne

2.Introduction to soil microbiology-Martin Alexander

3.Soil microorganisms and plant growth-N.S.Subba Rao

4 Advances in biogas technology-Chawla,O.P

Unit3

Microorganisms in soil fertility.Rhizosphere concept,R:S ratio ,Interaction between plant and rhizosphere flora.Growth promoting rhizobacteria,soil enzymes,Biocheleters(siderophore).Bifertilizers-Types and importance

.

References

1.Introduction to soil microbiology-Martin Alexander

2.Soil microorganisms and plant growth-N.S.Subba Rao

3.Soil microorganisms and plant growth  Somani.L.L and Bhandari,S.C

Unit 4

Biological nitrogen fixation –Nitrogenase enzyme-nif genes-symbiotic nitrogen fixation (Rhizobia,Frankia)-non symbiotic nitrogen fixation(azotobacter,Azospirillum).VAM-ecto-endo-ectendomycorrhizae.

References

1.Soil microorganisms and plant growth-N.S.Subba Rao

2 Biofertilizers-Somani etal

3 .Soil microorganisms and crop growth  Somani.L.L and Bhandari,S.C

4.Biofertilizers in agriculture-Subba Rao,N.S

Core Course- 9

MB3B09U Food Microbiology

Total hours- 72

Hours / week- 4

Credits- 4

Unit I:

History of food microbiology. Food as a substrate for micro organisms -. Micro organisms important in food microbiology; Molds, yeasts and bacteria - General Characteristics - Classification and importance.

Principles of food preservation - Asepsis - Removal of micro organisms, anaerobic conditions - High temperature - Low temperature - Drying - Food additives.

References

Frazier, W.C. 1978. Food Microbiology. McGraw Hill

Modern Food Microbiology  by James M. Jay, Martin J. Loessner, and David A. Golden

Unit II:

Contamination and spoilage - vegetables and fruits, meat and meat products, milk and milk products - fish and sea food - Poultry, Spoilage of canned foods. Food borne infections, poisoning and intoxications Microbiological examination of food, Milk. HACCP- definition and principles (brief)

References

Frazier, W.C. 1978. Food Microbiology. McGraw Hill

Modern Food Microbiology  by James M. Jay, Martin J. Loessner, and David A. Golden

Unit III:

Importance of microbes in food industries. Fermented food products by microbes – Bread, Vinegar etc. Alcoholic beverages- wine, beer, cedar. Oriental fermented foods. Fermented vegetables. Milk and mil products- butter, cheese, Probiotics (brief study)

Reference

Frazier, W.C. 1978. Food Microbiology. McGraw Hill.

Industrial Microbiology by Samuel C. Prescott

Parihar and Parihar Dairy Microbiology, Saraswati Purohit, Jodhpur India, 2007

Prajapati J. B. (1995), Fundamentals of Dairy Microbiology.

Unit IV:

Microbial cells as food-single cell proteins- Bakers yeast

Edible mushroom- types and production

Enzymes in food industry

References

Frazier, W.C. 1978. Food Microbiology. McGraw Hill.

Industrial Microbiology by L.E. Casida

Core Course 10 

MB3B10U  Microbiology Practical –III

Total hours of instruction: 72     

Hours / week : 4    

Credit : 2

 Enumeration of soil microbes by Plate culture method and isolation of

 microorganisms from soil sample – Bacteria, Fungi, Actinomycetes and

 Azotobacteria.

 Isolation of cellulolytic, Amylolytic, Lipolytic and Proteolytic Bacteria from Soil

 Wine Production from grapes

 Immobilization of yeast cells

 Study of Microbial contamination in food products

 Analysis of food samples- Vegetables, Fruits, Fish, Meat and processed food

 & isolation of bacteria and fungi.

 Milk analysis by SPC and MBRT

 Enumeration and isolation of Lactobacillus from curd

 Enumeration and isolation of Bacteria and mold from fermented foods

 Production of Alcohol from Cashew Apple

 Mushroom cultivation

   Reference

   Practical Microbiology by Dubey and Maheswari

   Experiments in Microbiology, Plant Pathology and Biotechnology by K.R. Aneja

   Text book of Biotechnology by R.C. Dubey

Core Course-11

MB4B 11U Aquatic microbiology

Total hours of instruction:72

Hours/week:4

Credits-4 

Unit 1

The aquatic environment and distribution of microorganisms in the aquatic environment. Aquatic ecosystem-fresh water(ponds,lakes,stream) marine(estuaries.mangroves,deep sea).Water zonations-upwelling.Benthic microorganisms.Marine microflora and biofouling

References

.Aquatic microbiology-Rheinheiner

.Marine pollution-Clark

Unit 2

Techniques for the study of aquatic microorganisms.The role and importance of aquatic microbial ecosystem.microbial consortia.Surface attachment and biofilm development.Antibacterial and bioactive compounds from aquatic microorganisms

References

Elements of microbiology- Pelczar,Reid and chan

Aquatic microbiology-Rheinheiner

Unit 3

Water pollution, microbial changes induced by inorganic and organic pollutants.metals as pollutants.algal blooms.biological and chemical control of algal blooms

References

.Elements of microbiology- Pelczar,Reid and chan

.Aquatic microbiology-Rheinheiner

.Fundamentals of bacteriology-A.J.Salle

 Ecological aspect of waste water treatment vol 2 biological activities      and  treatment process-Cruds C.R   and hawkes

 Microbiology-Prescott,M.J;harley,j.p.and klein,D.A

Unit 4

Potability of water.Purification and disinfection.indicator organisms.microbiological examination of drinking water.Water born diseases and control measures

References

 Elements of microbiology- Pelczar,Reid and chan

 Aquatic microbiology-Rheinheiner

 Fundamentals of bacteriology-A.J.Salle

 Ecological aspect of waste water treatment vol 2 biological activities    and  treatment process-Cruds C.R  and hawkes

 Microbiology-Prescott,M.J;harley,j.p.and klein,D.A

.Industrial microbiology-Casida,L.E

Core Course-12

MB4B12U Environmental microbiology

Total hours of instruction: 90

Hours/week:5

Credits:4

Unit 1

Concepts of microbial ecology .Microorganisms in the natural environment;Extreme environments and their adaptations.Significance of microbial activities in the environment

References

.Environmental microbiology-Vijaya Ramesh

.Introduction to environmental microbiology-Mitchel,R

.Soil microbiology-Waksman S.A

.Microbial ecology-Atlas and Bartha

 Unit 2

Aerobiology,.Sources of microorganisms in the air and their quantification techniques. Methods of sampling air.Factors affecting the extent and type of air microflora

References

 Environmental aspects of microbiology-Joseph C. Daniel

.Environmental microbiology-Vijaya Ramesh

.Introduction to environmental microbiology-Mitchel,R

.Microbial ecology-Atlas and Bartha

Unit 3

Biosensors and biological indicators. Waste water management and sewage treatment.Bioreactors for waste treatments.Biosurfactants.Biodeterioration of industrial products such as textiles ,paper, pulp,wood and paints

References.

General microbiology-Pelczar, Reid andChan

 Microbiology-Prescott,M.J;harley,j.p.and klein,D.A

 Biology of microorganisms-T.D.Brock

.Foundations in microbiology-Talaro,K and Talaro,A

. Microbiology:an introduction-   Tortora,G.j,Funke.B.R. and Case C.L

.Microbial ecology-Atlas and Bartha

Environmental molecular Microbiology:Protocols and applications by Paul A.Rochelle

Industrial Biotechnology by     Abhilash s.Mathuriya Ane books

Unit 4

Bioremediation and strategies for bioremediation.Phytoremediation Microbial degradation of petroleum and petroleum products,Pesticide degradation..Biostimulation and bioaugmentation.Fate of engineered microorganisms in the environmentMicrobial leaching and metal recovery.Microbes in mining and enhanced oil recovery.

References

Pesticide microbiology-Hill,I.R.andWright,S.J.l

Petroleum microbiology-Atlas.R.M

Microbiology-Pelczar.chan and Kreig

Bioremediation-Baker K.H and Herson                                                                                        

Biodegradation and bioremediation-Martin Alexander

Environmental microbiology-Vijaya Ramesh

Introduction to environmental microbiology-Mitchel,R

Microbiology fundamentals and applications-Ronald.M.Atlas

Core Course-13

MB4B13U Agricultural microbiology

Total hours of instruction: 72

Hours/week:4

Credits:4

Unit1

Brief account of microbial interactions –symbiosis-mutualism-commensalism-Amensalism-synergism-parasitism-predation. Plant –microbe and Animal-microbe interactions

References

.Microbiology Fundamentals and applications-Ronald M.Atlas

 Soil microorganisms and plant growth-N.S.Subba Rao

.Agricultural microbiology- G.Rangaswamy and D.J. Bagyaraj

.Microbial ecology-Atlas and Bartha

General microbiology-Pelczar, Reid andChan

 Microbiology-Prescott,M.J;harley,j.p.and klein,D.A

Unit 2

Microbial  diseases  of plants-common bacterial, fungal and viral plant pathogens. Transmission  and control measures.Natural defence mechanisms of plants. Microbial warfare on plants

References

Soil microorganisms and plant growth-N.S.Subba Rao

 Plant microbiology-. –campell,R

Agricultural microbiology- G.Rangaswamy and D.J. Bagyaraj

Advances in Agricultural microbiology-subba Rao

 Diseases of crop plants in India-G.Rangaswamy.

 Crop diseases and their management-Chaube  H.S.and Pundhir V.S

Unit 3

Phyllosphere microflora and its importance.Endophytic micrflora. Microboilogy of wood and silage,Tobacco curing. Role of microbes in retting  Rumen microbiology

References

1.Soil microorganisms and plant growth-N.S.Subba Rao

2. Plant microbiology-. –campell,R

3..Agricultural microbiology- G.Rangaswamy and D.J. Bagyaraj

4.Advances in Agricultural microbiology-subba Rao

5.Microbiology Fundamentals and applications-Ronald M.Atlas

Unit 4

Biopesticides-Bacterial-viral-fungalpesticides  Biological control of plant   diseases.Integrated pest management

References

1 Soil microorganisms and plant growth-N.S.Subba Rao

2. Plant microbiology-. –campell,R

3.Agricultural microbiology- G.Rangaswamy and D.J. Bagyaraj

4.Advances in Agricultural microbiology-subba Rao

5.Microbiology Fundamentals and applications-Ronald M.Atlas

6.Microbial ecology-Atlas and Bartha

7.General microbiology-Pelczar, Reid andChan

8 Microbiology-Prescott,M.J;harley,j.p.and klein,D.A

Core Course 14

MB4B 14U   Microbiology Practical – IV

Total hours of instruction: 72     

Hours / week : 4     

Credit : 2

 Enumeration and isolation of microorganism from fresh water and marine

    system.

 Microbial investigation of drinking water samples, total bacteria count, coliform

     test – MPN, Membrane Filter Technique.

 Estimation of BOD.

 Study of common Plant pathogen

 Isolation of pathogen- Citrus Canker , wilt.

 Estimation of rhizosphere microbial population  and calculation of R:S ratio.

 Isolation of nitrogen fixing bacteria – Rhizobium, Azospirillum, Azotobacter

 Reference.

 Experiments in Microbiology, Plant Pathology and Biotechnology by K.R. Aneja

 Microbiology Laboratory Manual by Cappuccino Sherman

Core Course- 15

MB5B 15U Medical Bacteriology- I

Total hours- 90

Hours / week- 5

Credits- 4

Detailed study of Morphology, Cultural characteristics, Biochemical, Epidemiology, Pathogenesis, Laboratory diagnosis, Prophylaxis and Treatment of the following bacteria:

Unit I:

Staphylococcus, Streptococcus

Reference:

.Mackie and Mc Carteny Practical Medical Microbiology – 13^th Edition, Churchill Livingstone.

.Ronald M.Atias (1989). Microbiology, Fundamentals and Applications. II edition. Maxwell Macmillan   International editions.

.David Greenwood, Richard C.B.Stack and John Forrest Peutherer. (1992). Medical Microbiology. 14^th edition. ELBS with Churchill Livingstone.

 Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K. Jayaram Panikar

Unit II:

Pneumococcus, Neisseria

Reference:

1.Mackie and Mc Carteny Practical Medical Microbiology – 13^th Edition, Churchill Livingstone.

2.Ronald M.Atias (1989). Microbiology, Fundamentals and Applications. II edition. Maxwell Macmillan International editions.

3.David Greenwood, Richard C.B.Stack and John Forrest Peutherer. (1992). Medical Microbiology. 14^th edition. ELBS with Churchill Livingstone. .

4..Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K. Jayaram Panikar

Unit III:

Corynebacterium, Bacillus, Clostridium

Reference:

.Mackie and Mc Carteny Practical Medical Microbiology – 13^th Edition, Churchill Livingstone.

Ronald M.Atias (1989). Microbiology, Fundamentals and Applications. II edition. Maxwell Macmillan International editions. .

Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K. Jayaram Panikar

Unit IV:

Enterobacteriacea I- Coliforms, Proteus

Enterobacteriacea II:- Shigella, Salmonella

Reference:

.

.Ronald M.Atias (1989). Microbiology, Fundamentals and Applications. II edition. Maxwell Macmillan International editions.

.David Greenwood, Richard C.B.Stack and John Forrest Peutherer. (1992). Medical Microbiology. 14^th edition. ELBS with Churchill Livingstone. 

Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K. Jayaram Panikar

Unit V:

Vibrio, Pseudomonas, Haemophilus

Reference:

Mackie and Mc Carteny Practical Medical Microbiology – 13^th Edition, Churchill Livingstone.

.Ronald M.Atias (1989). Microbiology, Fundamentals and Applications. II edition. Maxwell Macmillan International editions.

.David Greenwood, Richard C.B.Stack and John Forrest Peutherer. (1992). Medical Microbiology. 14^th edition. ELBS with Churchill Livingstone. .

.Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K. Jayaram Panikar

Core Course- 16

MB5B16U Medical Bacteriology- II

Total hours-72

Hours / week-4

Credits- 4

Detailed study of Morphology, Cultural characteristics, Biochemical, Pathogencity, Epidemiology, pathogenesis, laboratory diagnosis, prophylaxis and treatment of the following bacteria

Unit I:

Mycobacterium tuberculosis, M. leprae, Atypical mycobacteria, Actinomyces

 Reference:

1..Ronald M.Atias (1989). Microbiology, Fundamentals and Applications. II edition. Maxwell Macmillan International editions.

2..David Greenwood, Richard C.B.Stack and John Forrest Peutherer. (1992). Medical Microbiology. 14^th edition. ELBS with Churchill Livingstone. 

3.Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K. Jayaram Panikar

Unit II:

Bordetella, Brucella, Yersinia, Pasteurella, Francisella

1.Mackie and Mc Carteny Practical Medical Microbiology – 13^th Edition, Churchill Livingstone.

2.Ronald M.Atias (1989). Microbiology, Fundamentals and Applications. II edition. Maxwell Macmillan International editions.

3.David Greenwood, Richard C.B.Stack and John Forrest Peutherer. (1992). Medical Microbiology. 14^th edition. ELBS with Churchill Livingstone. .

4..Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K. Jayaram Panikar

1.      .

Unit III:

Spirochetes, Mycoplasma

Reference:

.Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K.  Jayaram Panikar

Mackie and Mc Carteny Practical Medical Microbiology – 13^th Edition, Churchill Livingstone.

Ronald M.Atias (1989). Microbiology, Fundamentals and Applications. II edition. Maxwell Macmillan International editions.

David Greenwood, Richard C.B.Stack and John Forrest Peutherer. (1992). Medical Microbiology. 14^th edition. ELBS with Churchill Livingstone.

Topley / Wilson’s (1990). Principles of Bacteriology, Virology and Immunity, VIII edition, Vol..III Bacterial Diseases, Edward Arnold, London.

Unit IV:

Rickettsiaceae, Chlamydiae

Reference:

.Mackie and Mc Carteny Practical Medical Microbiology – 13^th Edition, Churchill Livingstone.

Ronald M.Atias (1989). Microbiology, Fundamentals and Applications. II edition. Maxwell Macmillan International editions.

.David Greenwood, Richard C.B.Stack and John Forrest Peutherer. (1992). Medical Microbiology. 14^th edition. ELBS with Churchill Livingstone. .

.Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K. Jayaram Panikar

Core Course 17

MB5B 17U Medical Mycology

Total hours of instruction: 72     

Hours / week : 4    

Credit : 3

Unit I

General Characters of Fungi- Yeast and mold.Nutrition in Fungi.Cultivation of Fungi – Culture media and cultural characters.Methods for isolation of fungi. Staining methods used in mycology – wet mount and differential staining.Study of microscopic morphology – Ultra structure of yeast.

Reference

Text book of medical mycology  - Jagadish Chander , Interprint, New Delhi.

 Introduction to Mycology- Alexopolus.

 Botany for Degree students Fungi – B R Vashishta , A K Sinha

Unit II

Reproduction in Fungi- Asexual and Sexual method  Classification of Fungi – Principles and Approaches. Antifungal agents- Mechanism of action

Reference

 Text book of medical mycology  - Jagadish Chander , Interprint, New Delhi.

 Botany for Degree students Fungi – B R Vashishta , A K Sinha

Unit III 

Fungal Diseases: Causative Fungi, Clinical Manifestations, Laboratory Diagnosis and treatment – Brief account.

Superficial mycoses .Pityriasis versicolor , Dermatophytoses, Piedra

Subcutaneous mycoses – Mycetoma, Rhinosporidiosis, Phycomycosis, Sporotrichosis

Reference:

 Text book of medical mycology  - Jagadish Chander , Interprint, New Delhi.

 Mycology and virology – Topley and Wilson. Volume 4

 Medical Mycology by Rippon . W B Saunders. Co

 Manual of Clinical Mycology by Conant, Smith,Baker,Callaway & Mertics

Core Course 18 

MB5B 18U  Medical Parasiotology

Total hours of instruction:72

Hours/week:4

Credit:3

Unit I

Parasitology – General Concepts – Introduction to Parasitology, Classification – Host parasite relationship.                                          

Laboratory technique in Parasitology _ Examination of faeces for ova and cysts – Verm burden. Concentration methods, Floatation, Sedimentation techniques, staining by iron heamotoxylin methods, Blood smear examination – thick / thin Smears – Cultivation of protozoal parasites.

Reference 

 Text book of Parasitology by Jayaram Panickar

 Bailey and Scott’s Diagnostic Microbiology

 Practical Medical Microbiology – Mackie, McCartney

 Text book of Medical Parasitology by Parija S.C.

Unit II                     

Protozoology: Pathogenic mechanisms, Disease transmissions and their life cycles – Entamoeba and human disease , Plasmodia, Leishmania, Trypanosoma, Giardia,Trichomonas, Balantidium, Toxoplasma, Cryptosporidium and other protozoan parasites causing human infections. Influence of parasitic infections on immunocompromised hosts.

 Reference

 Parasitology by K.D. Chatterjee

 Text book of Parasitology by Jayaram Panickar

 Text book Medical Parasitology by Parija S.C.

 Text book of Medical Parasitology by  P. Chakraborty

Unit III 

Helminthology: Classification, Cestodes – Taenia. solium ,T. saginata, T.echinococcus , trematodes – Fasciola hepatica, Fasciolopsis buskii , Paragonimus westermanii, Nematodes – Ascaris , Schistosomes, Anchylostoma, Trichuris, Trichinella, Enterobius, Strogyloids and Wuchereria their life cycle , Transmission, pathogenicity and Lab Diagnosis.

Reference:

Text Book of Medical Parasitology by  P. Chakraborty

Text Book of Parasitology By Jayaram Panicker

Text Book of Medical Parasitology by Parija  S.C.

Core Course-19

MB5B 19  Microbiology  Practical – V

Total hours of instruction: 90          

Hours/week: 4          

Credit: 2

General procedure for the systematic study of Bacteria – Morphology, 

Staining, Colony Characteristics on BA ,MA,other selective Media.

Biochemical reactions of Bacteria: Sugar Fermentation , IMVIC ,H₂S

production,urease,Catalase, Oxidase ,TSI

Identification of Bacteria- Staphylococuus, E.coli, Klebsiella, Pseudomonas,

 Proteus, Salmonella typhi, Shigella, Vibrio cholerae.

Cultivation of Fungi- Study of colony characters of yeasts and Molds.

Microscopic morphology of molds- Pencillium, Aspergillus. Mucor, Rhizopus,

Fusaruim , etc by Lactophenol cotton blue mount examination.

Gram staining of yeast.

 Examination of Germ tube – Candida albicans

Reference:

 Bailey and Scott’s Diagnostic Microbiology

 Text book of Microbiology – Ananthanarayanan and Jayaram – Orient

 Longman

 Text book of Medical Mycology – Jagadish Chander . Interprint

 Practical Medical Microbiology by Mackie McCartney

 Manual for identification of Medical Bacteria by. S.T. Cowan

Core course 20                                                                    

MB6 B20U Medical Virology

Total hours of instruction: 72                                                   

Hours per week : 4

Credit:3

Unit 1

General characters of viruses – structure, classification, multiplication, cultivation.  Structure and replication of bacteriophages

 References

Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K. Jayaram Panikar

Belshe RB Textbook of Human Virology St. Louis: Mosby Year Book.

Dimock & Primrose Introduction to modern virology Oxford: Blackwell

White & Fenner Medical Virology New York : Academic Press

Collier & Oxford Human Virology London Oxford University Press

Fields et al Virology Philadephia: Lippincott – Raven

Zuckerman Clinical Virology Chichester : JohnWiley.

Topley & Wilson. Principles of Bacteriology, Virology and Immunity, VIII edition, Vol.III Bacterial Diseases, Edward Arnold, London.

Unit 2:

Characters and pathogenic significance of pox viruses, herpesviruses, picorna viruses & myxoviruses

 References

Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K.   Jayaram Panikar

Belshe RB Textbook of Human Virology St. Louis: Mosby Year Book.

Dimock & Primrose Introduction to modern virology Oxford: Blackwell

White & Fenner Medical Virology New York : Academic Press

Collier & Oxford Human Virology London Oxford University Press

Fields et al Virology Philadephia: Lippincott – Raven

Zuckerman Clinical Virology Chichester : JohnWiley.

Topley / Wilson’s (1990). Principles of Bacteriology, Virology and Immunity, VIII edition, Vol.III

Bacterial Diseases, Edward Arnold, London.

Unit 3:

Characters and pathogenic significance of arboviruses, rhabdoviruses, hepatitis viruses, oncogenic viruses & HIV

References

.Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K. Jayaram Panikar

Belshe RB Textbook of Human Virology St. Louis: Mosby Year Book.

Dimock & Primrose Introduction to modern virology Oxford: Blackwell

White & Fenner Medical Virology New York : Academic Press

Collier & Oxford Human Virology London Oxford University Press

Fields et al Virology Philadephia: Lippincott – Raven

Zuckerman Clinical Virology Chichester : JohnWiley.

Topley / Wilson’s (1990). Principles of Bacteriology, Virology and Immunity, VIII edition, Vol.III Bacterial Diseases, Edward Arnold, London.

Unit 4:

Diagnosis of viral infections.  Immunoprophylaxis and chemoprophylaxis of viral infections.

References

Ananthanarayan and Paniker's Textbook of Microbiology R. Ananthanarayan, C.K. Jayaram Panikar

Belshe RB Textbook of Human Virology St. Louis: Mosby Year Book.

Dimock & Primrose Introduction to modern virology Oxford: Blackwell

White & Fenner Medical Virology New York : Academic Press

Collier & Oxford Human Virology London Oxford University Press

Fields et al Virology Philadephia: Lippincott – Raven

Zuckerman Clinical Virology Chichester : JohnWiley.

Topley / Wilson’s (1990). Principles of Bacteriology, Virology and Immunity, VIII edition, Vol.III

Bacterial Diseases, Edward Arnold, London.

Core Course 21

MB6 B 21U Diagnostic Microbiology

Total hours of instruction: 90

Hours/week:5

Credits:4

Unit 1.

Safety in Clinical Microbiology  laboratory. Laboratory associated infections. Classification of microorganisms on the basis of hazards. Good laboratory practices. Microbiological safety cabinets- Types. Disinfection and decontamination of laboratory wastes. WHO safe code of practice for a clinical microbiology laboratory. Guidelines for the collection and transport of specimens

References

.Medical Laboratory  Manual  For Tropical Countries  Vol.II Microbiology.     MonicaCheesbrough ELBS

 Bailely &Scott  Diagnostic Micribiology.E.J. Baron, L.R.Peterson and S.M .Finegold .Mosby

Unit 2.

Scheme for the microbiological examination of sputum, throat and mouth specimens, nasopharyngeal swabs and aspirates, ear discharges, eye specimens, CSF, blood and bone marrow

References

Medical Laboratory  Manual  For Tropical Countries  Vol.II Microbiology. Monica Cheesbrough ELBS

Unit 3.

Microbiological examination of stool specimens, urine, urogenital specimens, skin specimens, pus from wounds, abcesses, burns and sinuses, and effusions.Ref:

Medical Laboratory  Manual  For Tropical Countries  Vol.II Microbiology. Monica Cheesbrough ELBS

Unit 4

Serological diagnosis: Applications of serological techniques such as agglutination reactions, precipitation reactions, complement fixation tests, and enzyme immunoassay for the diagnosis of Bacterial viral and immunological diseasesMolecular techniques in Microbiology- principles and applications Hybridisation, PCR, RFLP

References

.Mackie& McCartney Practical Medical Microbiology J.G.Collee,  A.G.Fraser,B.P.Marmion and A.Simmons (Eds.) Churchill Livingstone

Manual of Clinical  Microbiology P.R. Murray,E.J.Baron, J.H.Joorgenson M.A.Pfaller and Yolken R.H. ASM Press Washington DC

Unit 5

Laboratory control of antimicrobial therapy Antibiotic sensitivity tests. Disc diffusion and dilution methods, Determination of MIC and MBC. Antibiotic assays in body fluids..  Care and management of laboratory animals. Legal requirements for animal experiments. General aspects of organization of animal experiments- Preparation of animals, common experimental procedures, Humane methods of killing animals. Handling of common laboratory animals: Rabbit, guinea-pig, mouse and rat 

 References

 Medical Laboratory  Manual  For Tropical Countries  Vol.II Microbiology. Monica Cheesbrough ELBS

Ananthanarayanan and Paniker  Textbook of  Microbiology Orient Longman

Choice Based Course

MB6 B22AU Microbioprocess

Total hours of instruction 72

Hours per week 4

Credits: 4

Unit 1.

 Typical bioprocess. Different stages in bioprocess. Advantages of bioprocess over chemical process. Industrially important microbial metabolites

References

1.Microbial biotechnology-Principles and Applications L.Y.Kun (Ed.) World  Scientific

2.Modern concepts of Biotechnology H.D.Kumar Vikas Publishing Co. Pvt. Ltd. New Delhi

Unit 2.

 Isolation, screening and selection of industrially important microorganism. Mode of culturing- Batch, Continuous and  Fed-batch culture. Specific growth rate and yield

References

1.Industrial Microbiology Casida Jr.Wiley Eastern Ltd.

2.General Microbiology Vol.II Powar&Daginawala Himalaya Publishing House

Unit 3.

 Designing of media for fermentation. Defined and undefined media, Factors affecting fermentation. Optimisation of bioprocess. Precurssors, inducers, inhibitors, antifoam agents Submerged and solid state fermentation.

References

1.Industrial Microbiology Casida Jr.Wiley Eastern Ltd.

2.Industrial Microbiology – An Introduction  M.J.Waites ,N.L.Morgan, J.S.Rockey & G. Higton Blackwell Science

Unit 4.

 Bioreactor. Parts of bioreactor. Instrumentation of bioreactor. Aerobic microbioprocess. Importance of dissolved  oxygen. Volumetric oxygen transfer coefficient. Agitation. Aeration.

References

Microbial biotechnology-Principles and Applications L.Y.Kun (Ed.) World  Scientific

Industrial Microbiology – An Introduction  M.J.Waites ,N.L.Morgan, J.S.Rockey & G. Higton Blackwell Science

Unit 5.

Downstream processing- methods. Seperation of biomass. Purification of the products-various techniques. Economics and market potentials of fermentation products

References

1.Industrial Microbiology Casida Jr.Wiley Eastern Ltd.

2.Microbial biotechnology-Principles and Applications L.Y.Kun (Ed.) World  Scientific

Choice Based course

MB6B22BU Sanitation Microbiology

Total hours of instruction: 72

Hours/week:4

Credits:4

Unit 1

 General concept of sanitation and disinfection .Sanitation of industrial and food processing units Air sanitation,Safe location of animal houses,hospitals ,industrial fermentation units etc based on air sanitation.Air borne diseases and preventive measures.Biological weapons,their regulation and precaution.

References

 Fundamentals of bacteriology-A.J.Salle

 Microbiology-Prescott,M.J;harley,j.p.and klein,D.A

.Biology of microorganisms-T.D.Brock

Environmental aspects of microbiology-Joseph C. Danie

.Microbiology essentials and applications-Larry Mckane and Judy Kandel

Unit 2

Microbiology of  municipal sewage and sewage treatment.BOD and COD

Concept . Treatment of Industrial effluent-  Waste water treatment-Mechanical and biological. Aerobic and anaerobic treatments.Domestic septic tank.Treatment of municipal water supplies.water borne diseases

References

 Fundamentals of bacteriology-A.J.Salle

Ecological aspect of waste water treatment vol 2 biological activities and                                                                                                                                                                                                                              treatment process-Cruds C.R and hawkes

Microbiology-Prescott,M.J;harley,j.p.and klein,D.A

.Biology of microorganisms-T.D.Brock

Unit 3

Solid waste disposal-sanitary land fills,composting,vermicompost.Disposal of animal and agricultural waste.Methanogenesis and biogas production

References

 Fundamentals of bacteriology-A.J.Salle

 Ecological aspect of waste water treatment vol 2 biological activities and     treatment process-Cruds C.R and hawkes

 Microbiology-Prescott,M.J;harley,j.p.and klein,D.A

.Biology of microorganisms-T.D.Brock

Foundations in microbiology-Talaro,K and Talaro,A

Microbiology:an introduction-   Tortora,G.j,Funke.B.R. and Case C.L

 Industrial microbiology-Casida,L.E

Choice  Based Course

MB6 B22CU Medical Entomology

Total hours of instruction: 72               

Hours/week: 4                           

Credit: 4

Unit 1

Scope of Medical Entomology.Entomology and disease transmission.Modern concepts of    Entomology,Knowledge of the biology and life cycles of Arthropod vectors- metamorphosis.mechanism of disease transmission and types.Contol measures with particular reference to vectors and disease in India.

Reference:

Applied Entomology by P.G.Fenemore and Alka Prakash

Modern Entomology by D.B.Tembhare

General and applied Entomology by Nayar,Ananthakrishanan and David

Unit 2

Arthropods of Medical Importance-A brief account of the Biology,Life cycle,Mechanism of disease transmission and Control measures.

Class  Insecta-Mosquitoes flies-Sand fly,Tsetse fly,house fly,bed   bugs,louse,fleas

Reference

A hand book of Medical Entoamology and Elementary Parasitology by G.K.Rathnaswamy

 Hand book of Medical Entomology-EaswariNayar

Unit 3

Class Arachnida- Ticks (hard and soft), mites

Class Crustacea-Water fleas,crabs,Shrimps

Artropods producing toxic or allergic reactions in man-biting insects,spiders,scorpions.

Reference

 A hand book of Medical Entoamology and Elementary Parasitology by G.K.Rathnaswamy

 Hand book of Medical Entomology-EaswariNayar

 Modern Entomology by D.B.Tembhare

Unit 4

Entomological Techniques-Collection and maintenance for short periods for identification or isolation of pathogens.Preservation,labeling etc. for future use.

Reference

Applied Entomology by P.G.Fenemore and Alka Prakash

Hand book of Medical Entomology-EaswariNayar

Modern Entomology by D.B.Tembhare

Core Course -23

MB6 B23U Microbiology Practicals - VI

Total houre of instuction: 90          

Hours/week: 5          

Credit:3

 Isolation of pathogens from Clinical Speciments

 Identification of Pathogens- Bacteria

 Antimicrobial activity – Disc diffusion & broth dilution test, MIC

 Serological Test – WIDAL, RPR Card Test

Reference

 Medical Microbiology by Robert Cruickshank

 Bailey & Scott’s Diagnostic Microbiology

 Practical Medical Microbiology-Mackie & Mc Cartney

 Microbiology Laboratory Manual – Cappuccino Sherman

 Open Courses Offered by the Department

1. MB5 D01U Human Physiology (Credits:4)

2. MB5 D02U Bioinstrumentation and

    Techniques(Credits:4)

2.   MB5 D03U Environmental Science (Credits:4)

Open Course offered by the Department

MB5D 01AU Human  Physiology

Total hours of instruction :72

Hours per week: 4

Credits: 4

Unit 1.

 Elementary tissues- Epithelial, connective, muscular and  nervous tissue.    Homeostasis. Structure of different muscles. Mechanism of muscular contraction, Rigor mortis. Introduction to nervous system. Classification of nervous system. Synapse, ,Myoneural junction, Neurotransmitters

References:

Textbook of Medical Physiology.  Arthur C.Guyton and John E.Hall

.Essentials of  Medical Physiology. K.Sembulingam and Prema Sembulingam

Unit 2.

Composition and functions of blood, Haemoglobin, Blood clotting. Blood groups, Blood transfusion. Structure of heart. Action potential in cardiac muscle. Cardiac cycle. Heart sounds.            Conducting mechanism. Cardiac output Blood pressure, Pulmonary and systemic circulation. Organisation of respiratory system.  Pulmonary  ventilation. Gas transport. Control of respiration.

 References:

1.Textbook of Medical Physiology.  Arthur C.Guyton and John E.Hall

2.Essentials of  Medical Physiology. K.Sembulingam and Prema Sembulingam

Unit 3

Salivary and gastric glands  and their secretions. HCL secretion and regulation Pancreas structure and pancreatic juice. Structure and functions of liver.Bile.Kidney-structure and functions. Nephron. Renal regulation of water and  electrolyte balance

 References:

1.Textbook of Medical Physiology.  Arthur C.Guyton and John E.Hall

2.Essentials of  Medical Physiology. K.Sembulingam and Prema Sembulingam

Unit 4

Chemical classes of hormones. Action of hormones Endocrine glands- Hypothalamus, pituitory, thyroid, pancreas, adrenal and pineal gland. Role of reproductive  hormones, gastrointestinal hormones and  neurohormones Endocrine disorders

 References:

1. Endocrinology. Mac E. Hadley.

2 .Molecular Endocrinology. Fraklyn F.Bolander

Open Course offered by the Department

MB5D 02U  Bioinstrumentation and Techniques

Total hours of instruction: 72 

Hours/week: 4       

Credits: 4

Unit I

Colorimetry. Priciples and application 

Beer Lamberts Law, Turbidimetry, nephelometry, luminometry, Flame Photometer.

Reference

 Practical Biochemistry by Pattambiraman

 Biophysical Chemistry Principles and Techniques- Upadhyay, Nath.

Practical Biochemistry- Principles and Techniques - Ed Keith Wilson and John Walker       

Cambridge University press, Cambridge , U K.

Modern Experimental Biochemistry  Rodney F Boyer, The Benjamin /Cunnings 

 Publishing Company

Unit 2

Basic Principle and application of chromatography.

Paper, Gel. Thin layer, gas- liquid, ion exchange, affinity chromatography, H PLC.

Reference

Biophysical Chemistry Principles and Techniques- Upadhyay, Nath.

Practical Biochemistry  Principles and Techniques - Ed Keith Wilson and John Walker       

Cambridge University press, Cambridge , U K.

Modern Experimental Biochemistry  Rodney F Boyer, The Benjamin /Cunnings 

Publishing Company

Unit 3 

Basic Principles and application of centrifugation, Apparatus and procedures. Differential centrifugation , Density gradient centrifugation,Density gradient centrifugation.

Reference

 Biophysical Chemistry Principles and Techniques- Upadhyay, Nath.

Practical Biochemistry  Principles and Techniques - Ed Keith Wilson and John Walker       

Cambridge University press, Cambridge , U K.

Modern Experimental Biochemistry   Rodney F Boyer, The Benjamin /Cunnings 

Publishing Company

Unit 4

Basic  Principle and application of Electrophoresis

Gel Electrophoresis, PAGE, SDS PAGE , Paper. Two dimensional electrophoresis

Reference

Biophysical Chemistry Principles and Techniques- Upadhyay, Nath.

Practical Biochemistry- Principles and Techniques - Ed Keith Wilson and John Walker       

Cambridge University press, Cambridge , U K.

Modern Experimental Biochemistry - Rodney F Boyer, The Benjamin /Cunnings 

Publishing Company

Biochemical Methods- S. Sadasivam and  A. Manikam

Unit 5 

Spectrophotometry: Principles and application –UV, Visible and infrared Spectrophotometry  fluorescence spectrophotometry, AAS, flow cytometry

Reference

Biophysical Chemistry Principles and Techniques- Upadhyay, Nath.

 Practical Biochemistry” Principles and Techniques - Ed Keith Wilson and John Walker  Cambridge

University press, Cambridge , U K.

Modern Experimental Biochemistry  Rodney F Boyer, The Benjamin /Cunnings

Publishing Company

.Practical Biochemistry by Pattambiraman

Open Course  offered by the Department

MB5D 03U Environmental sciences

Total hours of instruction: 72

Hours/week:4

Credits:4

Unit 1